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Hi,  

On a widefield/FRAP setup, I want to combine a laser at 491 nm (fiber coupling) with an HBO xenon lamp (direct mount) on the back of my scope. The way things are arranged, I need to put a 45° dichroic mirror so that the lamp light will go through the mirror into the scope and the laser light will be reflected into the scope. So I'm looking for a dichroic mirror that would transmit everything (from 400 to 750) but a small window arround 491 nm. The window has to be narrow so that I don't loose too much GFP excitation light from the lamp. Is that kind of dichroïc available? I could'nt find one browsing Chroma or Semrock offers. Of course there is the option of having a custom one made, but I was wondering if someone had a link to an existing offer.

On a related note, I found lots of notch filters but not a lot of notch dichroics. What is the difference between a simple filter and a dichroic? What happens to the blocked light in the case of a filter, isn't it also reflected like for a dichroic?

Thanks for your help,

Christophe

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Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
http://www.cleterrier.net