There is also a spiffy new DNA dye in production that will excite with the
633 - it is called DRAQ5. The ref is: Smith et al. (2000) Cytometry
40:280-291 "Characteristics of a novel deep red/infrared fluorescent
cell-permenat DNA Probe, DRAQ5, in intact human cells analyzed by flow
cytometry, confocal and multiphoton microscopy"

I stumbled across this ref when I was looking for something else - oh,
happy day! The dye is not yet commercially available, but should be in
the near future (according to Dr. Smith).

Tamara Howard
CSHL


On Thu, 31 Aug 2000, Nicholas Terry wrote:

> Rosemary asked
>
> >What do people use the HeNe 633 laser for apart from Cy5?  If we got the
> >HeNe 594 it would replace the 633.
>
>
> To excite TOPRO-3 for nuclear staining. Allows use of a red channel for
> some marker other than PI or EB while saving having to use a UV laser to
> visualise Hoechst or DAPI.
>
> Regards,
> Nick
>
>
> Nicholas Terry, M.A., Ph.D.,
> Experimental Radiation Oncology - 066,
> The University of Texas M. D. Anderson Cancer Center,
> 1515 Holcombe Blvd., Houston, Texas 77030.
> 'Phone: 713-792-3424,
> 'Fax: 713-794-5369.
> http://drad52.mdacc.tmc.edu.
> [log in to unmask]
>