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Hi Jennifer,
You can see the spectrum here: 
http://www.mcb.arizona.edu/ipc/fret/index.html.  There are a number of 
filters/mirrors that you can check the spectrum against, also, and even 
calculate an estimated efficiency.
Cheers,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709
----- Original Message ----- 
From: "Jennifer Clarke" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Wednesday, June 13, 2007 6:07 PM
Subject: Spectrum Orange


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear all
>
> Can anyone please tell me the excitation and emission spectra for Spectrum
> Orange?
>
> I have a user who is interested in using Spectrum Orange for FISH, and we 
> would
> like to know if we can image this with any of our filter sets on our
> fluorescence microscopes, however I am having trouble finding out what the
> excitation and emission spectra for Spectrum Orange is.  Vysis, the/a 
> company
> that sells Spectrum Orange simply states that it can be imaged with their
> "Single Band Pass Orange Filter" (no filter specs provided, although they 
> sell
> the filters).
>
> (The only information on Spectrum Orange in the confocal list archives is 
> that
> the emission peak of Spectrum Orange is 10nm apart from Texas Red)
>
> Any comments on the photostability of Spectrum Orange, and any potential
> pitfalls of using this fluorophore, would also be appreciated
>
> Kind regards
> Jennifer Clarke
>
> -- 
> Jennifer Clarke BSc (Hons) PhD
> Confocal Microscopy Facility Manager
> Flinders Microscopy and Image Analysis Facility
> (c/- Department of Anatomy and Histology, School of Medicine)
> Flinders University
> GPO Box 2100, Adelaide 5001
> Phone: 61 8 8204 6454
> Email: [log in to unmask]