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Thanks to everyone for your suggestions about DRAQ-5 and mounting mediums.
I appreciate your input!

Robert Maher, Scientist
Pfizer Global Research and Development
e-mail:  [log in to unmask]



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]On
Behalf Of Jill Pflugheber
Sent: Wednesday, May 11, 2005 3:17 PM
To: [log in to unmask]
Subject: Re: DRAQ-5 and tissue sections


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Hi Robert,

We routinely use Draq 5 for rat brainstem sections, as well as ovarian
sections.  We mount with Fluormount G with good success.  The rat
brainstem sections are fixed in Streck and the ovarian section in % PFA.

I have noticed in our cultured cells, that acetone fixed cells don't
hold the Draq-5 label as well, but all the coverslips are fixed by
undergrads, so I don't know that they are really fixing long enough.

The Draq 5 works great for us.  I hope you get your problems solved.

Jill Pflugheber
Micrscopy Technician
St. Lawrence University
Canton, NY

Maher, Robert wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Does anyone have any experience with labeling sections with the nuclear
> probe DRAQ-5 (BioStatus Ltd.)?  I'm specifically interested in the
mounting
> medium chosen.  I labeled tissue sections with DRAQ-5 and mounted the
> sections with ProLong Gold (Molecular Probes).  After a day the staining
is
> significantly decreased.  I've also mounted the sections in water and
> glycerin and have had somewhat better success.  Any insight would be
> appreciated.
>
> Robert Maher, Scientist
> Safety Sciences Michigan-Ann Arbor
> Pfizer Global Research and Development
> e-mail:  [log in to unmask]
>
>
>
>
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