Sorry for the dual posting. I forgot to change the subject.
Dear users- What is the effect of not filling the back focal aperture
of an objective in a point scanning confocal system. I am assuming
the same laser power going in, but the beam not expanded as far. It
seems to me that it is a different issue than in a normal imaging
system. Would you get a smaller spot of illumination? Thanks- Dave
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"Home of the 2000 NCAA Women's Basketball Champions"
Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
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860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
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