CONFOCALMICROSCOPY Archives

May 2000

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Laser beam expansion and spot size
From:
David Knecht <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 24 May 2000 14:01:52 -0400
Content-Type:
text/plain
Parts/Attachments:
text/plain (22 lines) 
Sorry for the dual posting.  I forgot to change the subject.

Dear users- What is the effect of not filling the back focal aperture
of an objective in a point scanning confocal system.  I am assuming
the same laser power going in, but the beam not expanded as far.  It
seems to me that it is a different issue than in a normal imaging
system. Would you get a smaller spot of illumination?  Thanks- Dave
--

************************************************************
"Home of the 2000 NCAA Women's Basketball Champions"

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd.   U-125
Storrs, CT 06269
[log in to unmask]
860-486-2200      860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************

ATOM RSS1 RSS2