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Dear John,

We continue to use the older Leica NT (Excel-based) software here. If
you are also still using this version you can save the interesting part
of your focus series by:

1) entering "gallery" mode, then selecting (with mouse and
simultaneously pressed Ctrl key) each image of the part-series that you
want; (each selected image will have its frame boldly outlined).

2) use "File/save selected" to store the file. This writes out the part
series as "scanner file format", i.e. multiple-page TIFF.

3) open this newly-written file and you will now see a new gallery
containing only the images you selected.

This method is very useful for clearing away dirt and other bright but
unwanted things from uninteresting planes above and below the planes of
interest which would otherwise dirty-up the items of interest,
especially when projected in maximum intensity "proj" mode.
The method might also work with the later SP2 software, but I haven't
tried it out there.
Best

Jim

-----Original Message-----
From: John Runions [mailto:[log in to unmask]]
Sent: Friday, October 18, 2002 11:30 AM
To: [log in to unmask]
Subject: Re: z series


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Hi all,

Just a general comment/question on this topic.  It seems to me that
years
ago I could simply go to the gallery of images within a Zeiss Z-series,
select the portion of the series that I wanted to project and then
project
it.  Is this true?  The reason I ask is that that would seem to me to be
a
very useful feature.  I now use Leica confocal software and although I
have
mentioned this idea to several different engineers and sales people they
have always just looked at me funnily (mind you, a lot of people do
that) as
if this had never occurred to them.   What I do now is manipulation of
the
image stack as has been described for Biorad stacks.  Is there a simpler
way
to do this with Leica software?

John.

-------
C. John Runions, Ph. D.
Department of Plant Sciences
University of Cambridge
Downing St.
Cambridge UK
CB2 3EA

email: [log in to unmask]
phone: (01223) 766 545
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