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I mostly use MetaMorph, and also find the metadata from MetaMorph TIFF can
hardly be read by any other software packages.

However, I find the "legacy" stk files are more easily read (metadata-wise)
by all other software packages. So I changed the save preference to use
legacy stk format as default.

In MetaMorph journal front, I usually write a journal to loop through all
images in a folder, change the extension from tif to stk is sufficient to
force MetaMorph to re-save tif into stk format. I don't have MetaXpress, but
I suppose the journal can work in a similar fashion.

Best,

Tao

On Thu, Nov 18, 2010 at 4:54 AM, Mario Emmenlauer <[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Imaging List,
>
> this is my first post to this list, if it is off-topic or has been
> answered before please redirect me to the appropriate place! Thanks.
>
>
> I have a strange problem with MetaXpress image metadata. When I save
> images in the file format "MetaSeries Single-/Multi-Page TIFF", then
> Bio-Formats can read a lot more metadata (exposure time, stage coords,
> etc) then when saving as "MetaMorph TIFF" file format.
>
> I save images in MetaXpress from a Journal, using "Sequential File Names".
> But the Journal setup doesn't allow me to choose the "MetaSeries" file
> format, it falls back to "MetaMorph TIFF" wheneber I click "ok"! Is that
> a bug? We have a quite recent version (3.1.0.73).
>
>
> I found a workaround, by editing the Journal with a text editor. When
> I replace in Variable "uniqueSetupJNL.stImageType" the "4 .tif" with
> "5 .tiff", then MetaXpress will save as "MetaSeries Single-/Multi-Page
> TIFF" file format, and all metadata is there!
>
> This is an ugly hack, do you have a better solution to achieve this?
> Am I doing something wrong? I'm really new to high content screening
> and MetaXpress :-/
>
>
> All the best,
>
>    Mario
>



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