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Hi Sarah-

I see you've been getting lots of replies concerning different types
of plastic coverslips.

A possible alternative-  I seem to recall that there was a synthetic
protein available 4-5 years back that might help. I believe it went by
the name of Pronectin.

A search engine should help verify that,they had a technical paper
that should turn up in PubMed, for example. May be competing product
available by now as well.

It was engineered with multiple repeats of several conserved binding
domains from extracellular matrix proteins like Fibronectin and one of
its uses was coating glass coversilps for improved adhesion between
culture cells and glass.

Scott

J. Scott Gens
Post-doctoral Fellow
Department of Cellular & Integrative Physiology
Indiana University School of Medicine
Indianapolis IN

-------------------
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi-
> I have a couple of users who want to image cells grown in plastic
> microwell plates on an inverted scope.  Of course, the plastic is
too
> thick to image through, but when I suggested that they grow them on
> glass coverslips they replied that the cells don't stick as well and
> grow differently on similarly coated glass.  Is it possible to buy
> microwell plates with thinner plastic bottoms, and where would I buy
> such a thing?  In previous postings I got the feeling that the only
> available ones had glass coverslip bottoms.  Also, would the images
> suffer greatly when imaging through plastic versus glass?  Would a
> better option be to just get a longer-working distance lens?
> Thanks in advance-
> Sarah
>
> ------------------------------------------------------------
> Sarah Locknar, Ph.D.
> Director, Neuroscience COBRE Imaging/Physiology Core
> University of Vermont
> 802-656-0413
> ------------------------------------------------------------
>
>
>