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Hi,
IPLab software also controls all of these things. It is available on both
Mac and Win and through the use of easy to write macros you can create and
control multidimensional acquisitions.
Jim
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Jim Paladino, Vice President
BioVision Technologies, Inc.
80 West Welsh Pool Road, Suite 101N
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Edward Monosov, Ph.D.
Sent: Tuesday, May 04, 2004 6:22 PM
To: [log in to unmask]
Subject: Re: Perkin Elmer Spinning disk under Metamorph
Hi Steve,
Here is a copy of my own answer to Gabor related to my setup:
"I am running PE Ultraview spinning disk under MM (v.6.1.5) using Multi
Dimensional Acquisition (MDA) module, which controls:
a) all firmware of the inverted Olympus IX81 microscope including the
Z-motor, nosepieces, filter cubes and condenser;
b) the Sutter Lambda 2-10 controller for the excitation and emission filter
wheels, and for the Uniblitz shutter installed into the bright field
illuminator.
c) the Orca ER (Hamamatsu) CCD camera.
d) the X-Y stage (ASI);
All these elements are hooked up to the left side port of my Olympus; "
On the right side port I have another optical setup included the
"Dual-View" (Optical Insights), CCD camera "Cascade" (Roper) and DJ-4
(Sutter). Everything controls.by MetaMorph.
I would like to make a suggestion related to your worries about control of
the laser power. Probably, you have already found yourself that you can use
your MetaMorph for neutral density filters installed into filter wheels.
Ed
Steve Bagley wrote:
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Hi Gabor,
I have been chatting to Universal Imaging regarding exactly the same issue.
Being able to set up complex time lapse experiments/Macros, such as
capturing brightfield images and then a confocal image every so often would
be extremely beneficial. Sadly the Ultraview software seems a little lacking
in this respect.
I gather than MetaMorph can control camera, exposure/gain/capture and the
scan head but it would be difficult to control the laser power from
MetaMorph. If anyone have heard differently please let me know as I would
also benefit from a MetaMorph/Ultraview combination.
All the best,
Steve
Steve Bagley
Associate Scientist
Applied Imaging Facility
Paterson Institute For Cancer Research
Cancer Research UK
Christie Hospital, Wilmslow Rd,
Manchester. M20 9BX, UK
-----Original Message-----
From: Gabor Csucs [mailto:[log in to unmask]]
Sent: 03 May 2004 11:50
Subject: Perkin Elmer Spinning disk under Metamorph
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Dear All
For some reasons we would like (eventually) to run our Perkin Elmer
(UltraView) spinning disk system under Metamorph. I know that in
principle it is possible to control a spinning disk system with
Metramorph (e.g. Visitech) but is there anybody who has experience with
a Perkin-Elmer/Metamorph combination? I'd very much appreciate your
comments.
Thanks Gabor
--
Gabor Csucs PhD
Light Microscopy Centre ETH Zürich
Inst. of Biochemistry
ETH Hönggerberg HPM D 8.1
Tel:41-1-633-6221
e-mail: [log in to unmask]
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('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author and do not necessarily represent
those of the Paterson Institute for Cancer Research or the Christie Hospital
NHS Trust. It may contain information that is privileged & confidential
within the meaning of applicable law. Accordingly any dissemination,
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by any person other than the intended recipient may constitute a breach of
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--
Edward Monosov, Ph.D.
Director , Cell Imaging & Histology
The Burnham Institute
10901 N. Torrey Pines Rd,
La Jolla, CA 92037
Ph: (858)646-3100 ext. 3206
Fax: (858)646-3196
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