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February 1999

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Subject:
From:
Ammasi Periasamy <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 24 Feb 1999 08:33:39 -0500
Content-Type:
text/plain
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Hi Xuejun

There are two companies uses unix platform for data acquisition and digital
deconvolution. (1) Applied Precision (Deltavision) - 425-557-1000 and (2)
Inovision corporation 919-788-9998

I have these two companies software. Recently Inovision added spectral
bleadthrough corrections in their software.

good luck
Ammasi Periasamy, Ph.D.
---------

At 15:37 2/23/99 -0700, you wrote:
>Hi, Everyone:
>
>We are looking into a standalone 3-D image processing/analyzing workstation
>and a back illuminated CCD camera. The primary usage we need to perform on
>the workstation are:
>
>1) De-convolution.
>
>2) 3-d image processing/measurement. More specifically, we need to get
>integrated density measurement of 3-d/4-d (3-D time-laps) experiments. Also,
>possibly, we need to measure of co-localization of multiple stains in 3-d.
>
>We do have a PC based de-convolution package now. The problem is speed. Even
>with the fastest computer we can get and maximum amount of RAM we can put
>into, it is still fairly time consuming with the data set we have.
>
>The questions I have are:
>
>1) Any recommendations about particular software and computer platforms for
>all above applications? I know it is a difficult question. I am particularly
>interested in the experience of those who use a Unix platform for the above
>purposes. It is intended  for use in a multi-user facility.  Consequently,
>ease of use and flexibility of software is a main concern.
>
>2) If anyone has compared PC based and Unix based system for such
>applications, I would particularly like to know the pros and cons of the two
>platforms relative to each other.
>
>3)  If anyone has opinions on particular back illuminated CCDs.  Our key
>requirements are a) high sensitivity in the green wavelengths, b) small
>pixel size (no greater than 10 micron pixels), c) relatively fast frame
>acquisition with a greater emphasis on low dark current and readout noise
>
>I thank, in advance,  those who respond. Please respond to me directly
>instead of to the list (unless you think it is of generally interesting).
>
>Commercially motivated responses are welcome. Nevertheless, we are in the
>early stage of thinking whether it is worth to pursue further. So there is
>no definitive plan for this yet.
>
>Best wishes to all.
>
>Xuejun
>
>***********************************************************************
>Xuejun Sun, Ph.D.
>Dept. of Experimental Oncology
>Cross Cancer Institute
>11560 University Ave.
>Edmonton Alberta T6G 1Z2, Canada
>
>Phone:  (780) 432 8898 (office)
>        (780) 432 8468 (lab.)
>Fax:    (780) 432 8892
>Email:  [log in to unmask]
>
>
Ammasi Periasamy, Ph.D.
Director, W.M. Keck Center for Cellular Imaging
University of Virginia
Biology, Gilmer Hall (039)
Charlottesville, VA 22903
E-mail: [log in to unmask]
http://www.cci.virginia.edu/
Tel: (804) 243-7602; Lab.: (804) 982-4869; Fax: (804) 982-5210

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