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Dear Paul,
thank you very much for your advice, this sounds great. I will try it
out.
Best wishes,
Marianne
On May 18, 2005, at 6:45 PM, Paul Rigby wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Marianne,
> It is likely that the autofluorescence that you are getting in the
> pancreas is from lipofuschin deposits, particularly seen in slightly
> older animals or tissues. This can be quenched using Sudan Black
> staining or copper sulphate treatment, thereby enabling you to use the
> green fluorescence channel. We have previously used the Sudan Black
> treament in retinal wholemounts and sections and in cardiac tissue
> with excellent results. There is some basic methodological information
> (and references to the original publications) on our website:
>
> http://biaf.uwa.edu.au/biaf/autofluor.htm
>
> I hope this helps. Good luck.
> Paul Rigby
>
> ---------------
>
> Dr Paul J Rigby
> Director and Senior Lecturer
> Biomedical Imaging & Analysis Facility (M510)
> The University of Western Australia
> 35 Stirling Highway, Crawley, WA, 6009
> Australia
> Ph (61-8) 9346 2819
> Fx (61-8) 9346 3469
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]On
> Behalf Of Marianne Martinic
> Sent: Thursday, 19 May 2005 12:36 AM
> To: [log in to unmask]
> Subject: Tracking of live cells
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello everybody,
>
> I am just starting using the confocal microscopy and would like to ask
> if anyone of you already traced activated and labeled CD4 and CD8 T
> cells in the pancreas? Due to the high autofluorescence of the
> pancreas, I thought of chosing PHK26 from Sigma together with DiD from
> Invitrogen to label the T cells in order to avoid the green
> fluorescence channel.
>
> Thank you very much for any advice,
>
> Marianne
> --
> Marianne Martinic, Ph.D.
> La Jolla Institute for Allergy and Immunology
> Division of Developmental Immunology DI-3
> 10355 Science Center Drive
> San Diego, CA 92121
> U.S.A.
> Phone: (858) 678 4581
> Fax: (858) 558 3579
> [log in to unmask]
>
>
--
Marianne Martinic, Ph.D.
La Jolla Institute for Allergy and Immunology
Division of Developmental Immunology DI-3
10355 Science Center Drive
San Diego, CA 92121
U.S.A.
Phone: (858) 678 4581
Fax: (858) 558 3579
[log in to unmask]
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