This should not be any problem on (for example) a Prairie or LaVision system fitted on an Olympus stand. I really find it hard to believe that Olympus would not supply this lens for such use. Can anyone actually confirm that they will not? Is there anyone from Olympus on this list?
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
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Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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________________________________
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of RICHARD BURRY
Sent: Thursday, 14 May 2009 10:47 PM
To: [log in to unmask]
Subject: Re: Recommendations for commercial multi-photon system purchase
The Olympus 25x 1.05 N.A. objective has an enlarged aperture in the back image plane to allow more light throughput. This requires additional lenses capable of filling the aperture, which Olympus has. While the lens may fit on other microscopes, with ut the filling of this aperture, it will not perform as well as it could.
Dick
----- Original Message -----
From: Adrian Smith <[log in to unmask]>
Date: Thursday, May 14, 2009 8:02 am
Subject: Re: Recommendations for commercial multi-photon system purchase
To: [log in to unmask]
> I don't believe there is any technical reason why the 25x
> Olympus will
> not work on the LaVision BioTec or Prairie systems.
>
> However, my understanding is that Olympus will only sell
> that
> objective with a new Olympus MPE system... (which makes it a lot
> more
> than just "super expensive" :).
>
> Extremely disappointing decision on Olympus' part.
>
> I would absolutely love to try the objective on our Olympus
> microscope
> (supplied by LaVision BioTec) but I am unable to. If it performs
> as
> well as the marketing material suggests then I would be willing
> to pay
> a premium for it.
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
> On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:
>
> > If and when you try the olympus system make sure they bring
> you the
> > super expensive dipping/coverslip water optic (25X1.12 NA)
> which
> > they designed specifically for 2p. It is absolutely
> spectacular,
> > and pretty much the only lens we use for all our mpe work
> nowadays.
> > When you compare systems it may make you lean towards
> this
> > particular manufacturer. Of course the LaVision and
> Prairie scan
> > heads will fit on the Oly stand, not sure whether they will
> work
> > with this lens but it would be great to find out (anyone out
> there
> > tried this combo yet).
> > S.
> > Simon C. Watkins Ph.D, FRC Path
> > Professor and Vice Chair Cell Biology and Physiology
> > Professor Immunology
> > Director Center for Biologic Imaging
> > BSTS 225
> > University of Pittsburgh
> > 3500 Terrace St
> > Pittsburgh PA 15261
> > 412-352-2277
> > www.cbi.pitt.edu
> >
> > ________________________________________
> > From: Confocal Microscopy List
> [[log in to unmask]] On
> > Behalf Of Adrian Smith [[log in to unmask]]
> > Sent: Wednesday, May 13, 2009 8:04 PM
> > To: [log in to unmask]
> > Subject: Re: Recommendations for commercial multi-photon
> system
> > purchase
> >
> > On 14/05/2009, at 1:57 AM, Alison North wrote:
> >
> >> If you were only interested in multiphoton imaging, the
> LaVision has
> >> certain advantages, but if you need visible lasers too then I'm
> >> afraid it won't suit your purposes.
> >
> > I'm not sure of the details but the LaVision BioTec did
> mention some
> > confocal options to me in passing when we were discussing
> > specifications recently. We were not interested so I didn't
> ask any
> > details but it might be worth clarifying with them what they can
> > currently offer.
> >
> > From recollection I think the Prairie was also MP-only, at
> least when
> > we were looking (that was one of its selling points). Maybe
> that has
> > changed or I'm misrembering?
> >
> > Regards,
> >
> > Adrian Smith
> > Centenary Institute, Sydney, Australia
>
>
> --
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