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Hi Jason,
You might see what a Pathology text has to say about hemosiderin, a product
of hemoglobin/RBC breakdown by macrophages that would commonly be found at
sites of hemorrhage.  The timing seems about right for this.  With a mix of
modified ferritin, digested porphyrins, 30% iron and who knows what all, I'd
say the chances of autofluorescence are high.  As for quenching, I have no
ideas.
good luck,
Carl


Carl Boswell
Dept. of Molecular and Cellular Biology
Univ. of Arizona
520-626-8469
520-621-3709 FAX
----- Original Message -----
From: "Jason W" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Saturday, May 08, 2004 10:30 AM
Subject: Autofluorescence after intracerebral hemorrhage


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello,
>
> I'm a new member to the list so this is my first message. I am working on
a
> model of intracrebral hemorrhage in the rat. The hemorrhage is in the
> striatum. The rat is perfused with 4% para and then the brain is post
fixed
> for one night in 4% para and then 1 day in 10% sucrose and then 1 week in
30%
> sucrose. The brain is frozen in CO2 and then sliced in a microtome. I have
> observed a bright ring of autofluorescence around the hemorrhage 7 days
after
> hemorrhage onset. The autofluorescence is not visible in other parts of
the
> brain. The autofluorescence appears as small, punctate spots. They appear
> orange when excited by UV, green when excited by blue  and red when
excited by
> green. From what I have read they appear to be lipofuscin but I have not
found
> any literature discussing the presence of lipofuscin in intracrebral
> hemorrhage. Anyone have any experience with this and any suggestions for
> quenching it? Thank you very much.
>
> Jason Wasserman
> University of Toronto, Dept. of Physiology
> Toronto Western Research Institute
> Toronto Western Hospital
>
> --