Hi Andrea,

If you go to the Process menu and select Channel Shift, this will allow you
to move channels in relation to each other.  The Zeiss Manual should explain
everything you need to know.

What objective are you using when you experience this shift?  You may be
able to eliminate this shift by using a properly corrected objective for the
wavelengths you use.

Gook Luck!

-Jason

Jason Kirk
Center for Biomedical Imaging Technology
University of Connecticut Health Center
263 Farmington Ave.
Farmington, CT 06030
Ph: (860)679-4686
Fax: (860)679-1039
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-----Original Message-----
From: Confocal Microscopy List
[mailto:[log in to unmask]]On Behalf Of Andrea Murmann
Sent: Monday, November 05, 2001 10:24 PM
To: [log in to unmask]
Subject: pixle shift


Hi all,

I am working on a LSM 510 (Zeiss) and I have been collecting 3 color
z-sections (fitc, rhod, cy5) in "multitrack" mode. I have noticed a
significant pixel shift between the green and red channel. Since I am
interested in showing colocalisation and want to use the 3D function of the
program to calculate a projection, this shift is irritating. How can I
compensate for the shift while in the LSM 510 program so that I do not have
to go picture by picture in Photoshop?

Thanks in advance,
Andi

_________________________________________________________

Andrea E. Murmann

The University of Chicago
Department of Medicine
Section Hematology/Oncology (Lab I-312)
5841 South Maryland Avenue, MC 2115
Chicago, IL 60637-1470
U.S.A.

fax:    (773) 702-3163
phone:  (773) 834-1539

e-mail: [log in to unmask]