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This discussion is most opportune, Michal and Sabina...
We've been having a look at the Zeiss LSM 510 META system, and we were
really puzzled by the file format... in particular, if we tried to
image four or five colour fluorophores in a 3D stack (not worrying
about bleed through / discrimination issues for now...), using the META
detector to image two or three well separated fluorophores in addition
to the stand alone detectors, it was not at all obvious how to extract
the individual META channels as separate stacks that were not
preassigned to RGB channels... maybe I missed something in the demo.
But even a stack of TIFF files would be OK - is that how it works,
Michal?
any edification here for a confused BioRadder, gratefully received!
IAN
On Tuesday, May 3, 2005, at 09:26 PM, Sabina Muller wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Thank's Michal,
>
> but this gives us tiff files and we would like to have the original LSM
> files ...
> sam
>
>
> A 13:54 03/05/2005 +0200, vous avez écrit :
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Sabina Hi,
>> You have to export the files correctly from the LSM510 software.
>> Open the image you like, under "split mode". Under "export/image type"
>> open the "raw data single" and then choose which channel you would
>> like
>> to export, give each one a separate name.. And you have it.
>>
>> Good Luck
>> Michal
>>
>>
>> **********************************
>>
>> Michal Hershfinkel, PhD
>>
>> Department of Morphology, room 339
>>
>> Faculty of Health Sciences
>>
>> Ben Gurion University of the Negev, Beer-sheva, Israel
>>
>> Tel: 972-8-6477318
>>
>> Fax: 972-8-6477627
>>
>> **********************************
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[log in to unmask]]
>> On
>> Behalf Of Sabina Muller
>> Sent: Tuesday, May 03, 2005 12:36 PM
>> To: [log in to unmask]
>> Subject: Metamorph/LSM files
>>
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear confocalists,
>>
>> we are looking for some help regarding analysis of LSM files obatained
>> with a Zeiss LSM 510 confocal microscope by Metamorf software. We
>> can't
>> open immages aquired with 3 different fluorochromes. They sort in
>> black
>> and white if opened with the Metamorf software and there is no way to
>> retrive the original single information for each channel used. There
>> is
>> only one image. Does somebody know how to overcome this problem?
>> Than's
>> for your help sam
>>
>> Sabina Mueller Valitutti
>> Cell Imaging Unit
>> IFR30, CPTP
>> TOULOUSE
>> France
>>
>
>
* * * * * * * * * * *
Prof Ian Gibbins
Anatomy & Histology
Flinders University
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