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Hello,

I am not sure what kind of LSM files you have (3 channel in 3D or timelapse?
it can be really complex according to the set up of the instrument) and what
kind of analysis you want to do. But Metamorph does read LSM files WHEN it
is no more than 3 channels/file (either in single plane or 3-D format). It
will open it as RGB (which may show up in weird color as it just assigns RGB
according to the sequence of the channels). Then, you can split them into
tiffs in Metamorph as it has limited functions dealing with RGB files.
Alternatively, you can export it as tiff series in LSM and then open them in
Metamorph.

Hope this helps.

Xuejun


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Sabina Muller
Sent: Tuesday, May 03, 2005 4:36 AM
To: [log in to unmask]
Subject: Metamorph/LSM files

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Dear confocalists,

we are looking for some help regarding analysis of LSM files obatained with
a Zeiss LSM 510 confocal microscope by Metamorf software.
We can't open immages aquired with 3 different fluorochromes. They sort in
black and white if opened with the Metamorf software and there is no way to
retrive the original single information for each channel used. There is
only one image.
Does somebody know how to overcome this problem?
Than's for your help
sam

Sabina Mueller Valitutti
Cell Imaging Unit
IFR30, CPTP
TOULOUSE
France


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