Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Alan's book is very complementary to the others suggested. It is an
excellent introduction of topics and emphasizes the actual practice
of confocal microscopy over theory - e.g. finding your cells without
bleaching them, choosing the most appropriate system for a sample, etc.
Glen
On Jun 5, 2007, at 9:55 AM, Julio Vazquez wrote:
> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-
> bin/wa?S1=confocal
>
> Lorie,
>
> For a general introduction, I really like Douglas B Murphy,
> Fundamentals of Light Microscopy and Electronic Imaging (Wiley-Liss).
> I suspect (haven't had a chance to read it yet) the book by Alan
> Hibbs: Confocal Microscopy for Biologists (Springer) should be
> outstanding, and more focused on confocal microscopy.
>
>
> --
> Julio Vazquez,
> Fred Hutchinson Cancer Research Center
> Seattle, WA 98109-1024
>
>
> [log in to unmask]
> http://www.fhcrc.org/science/shared_resources/imaging/
>
>
>
>
> On Jun 5, 2007, at 9:45 AM, Loralei Dewe wrote:
>
>> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/
>> cgi-bin/wa?S1=confocal
>> So it sounds like I have more to learn...what books besides the
>> Pawley book would someone recommend?
>>
>> Cheers,
>> Lorie
>>
>> Live as if you were to die tomorrow.
>> Learn as if you were to live forever. - Gandhi -
>>
>>
>>
>> -----Original Message-----
>> From: Armstrong, Brian
>> To: [log in to unmask]
>> Sent: Tue, 5 Jun 2007 8:29 am
>> Subject: Re: Confocal without lasers
>>
>> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/
>> cgi-bin/wa?S1=confocal Hi, I would just add to this that the
>> pinhole must match the objective mag/na in order to be truly
>> confocal such that = 1 Airy Unit. I believe that Olympus
>> accomplishes this by providing several disks with different sized
>> pinholes (if I remember correctly). The classic Yokogawa spinning
>> disk is optimized for a 100x obj. You should refer to Pawley's
>> Handbook for a complete explanation, or for a quick explanation
>> see Wikipedia. Cheers, http://en.wikipedia.org/wiki/
>> Confocal_laser_scanning_microscopy Brian D Armstrong PhD Light
>> Microscopy Core Manager Beckman Research Institute City of Hope
>> 1450 E Duarte Rd Duarte, CA 91010 626-359-8111 x62872 http://
>> www.cityofhope.org/SharedResources/LightMicroscopy -----Original
>> Message----- From: Confocal Microscopy List
>> [mailto:[log in to unmask]] On Behalf Of Turan Erdogan
>> Sent: Tuesday, June 05, 2007 8:19 AM To:
>> [log in to unmask] Subject: Re: Confocal without lasers
>> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/
>> cgi-bin/wa?S1=confocal Dear Loralei: I would add that there is
>> nothing magical about the laser light, other than that due to its
>> "brightness" (watts per cm^2 per steradian per unit wavelength),
>> it is generally easier to focus more watts through a pinhole. But
>> as long as you can get enough light through the pinhole, even if
>> it comes from a broadband, less-coherent arc-lamp source, you can
>> still do excellent confocal imaging. In the case of the Olympus
>> system, they don't actually use a single pinhole, as in
>> conventional laser-scanning confocal systems, but rather a disk
>> with a series of crossed linear apertures, such that when the disk
>> spins the pattern of light that gets through the disk as a
>> function of time appears like an array of moving apertures (it is
>> a variation of the so-called "Nipkow disk" which contains an array
>> of actual pinholes arranged in a spiral pattern). These sorts of
>> spinning disk systems make more efficient use of the light than
>> single-pinhole, laser-scanning confocal systems, and therefore are
>> able to function quite well even with less-bright, non-laser
>> sources. Of course if you can get away without using lasers, you
>> gain the flexibility of having all wavelengths available to you,
>> plus compactness, less heat, etc., which is why it makes sense to
>> use a lamp if you can. Hope that helps a little more. Sincerely,
>> Turan Dr. Turan Erdogan, CTO Semrock, Inc. 3625 Buffalo Road
>> Rochester, NY 14624 tel: (585) 594-7001 fax: (585) 594-7095
>> [log in to unmask] www.semrock.com The Standard in Optical Filters
>> for Biotech & Analytical Instrumentation More than 100,000 Ion
>> Beam Sputtered filters delivered - extensive inventory now! -----
>> Original Message----- From: Peng Xi [mailto:[log in to unmask]]
>> Sent: Tuesday, June 05, 2007 11:11 AM To:
>> [log in to unmask] Subject: Re: Confocal without lasers
>> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/
>> cgi-bin/wa?S1=confocal Hi, The core for confocal is: the
>> excitation source focal spot and the detection focal spot matches
>> each other via the target focal spot. Then, if you put sample onto
>> the target spot, the detector detects the energy only from this
>> spot and rejects adjacent. So the key for excitation is to make a
>> spot. A laser, is an ideal source to do that. On the other hand,
>> any pinhole (or a pinhole array) can be a point source. Of course,
>> you have to match the excitation pinhole(s) to the detection
>> pinhole(s) via the target pinhole images. In the case of Olympus
>> Spinning disk, it has an epi-illumination so the excitation and
>> detection uses the same pinholes and they line up together
>> automatically. Wish this helps! Peng -- Sincerely, Peng Xi Dantus
>> Research Group Department of Chemistry Michigan State University
>> East Lansing, MI 48824 Tel: (517) 355-9715 x319 Email:
>> [log in to unmask] http://www.msu.edu/~xipeng/ Loralei Dewe
>> wrote: > Search the CONFOCAL archive at > http://
>> listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > Hi All, > > I
>> may be putting my foot in my mouth but I do not understand how a >
>> system can be a true confocal without lasers. We just purchased an
>> > Olympus Spinning Disk system which uses a Mercury lamp and
>> filters. I > thought it was by virtue of the lasers being able to
>> penetrate the > tissue and focus on all the layers making a 3-D
>> image that made a > Confocal but the Olympus rep swears theirs IS
>> a true confocal. Can > someone explain this to me for my
>> education? > > Thanks > > Loralei Dewe > > > >
>> ---------------------------------------------------------------------
>> - > -- > See what's free at AOL.com > <http://www.aol.com?
>> ncid=AOLAOF00020000000503>. "EMF " made the following annotations.
>> ---------------------------------------------------------------------
>> --------- SECURITY/CONFIDENTIALITY WARNING: This message and any
>> attachments are intended solely for he individual or entity to
>> which they are addressed. This communication may contain
>> information that is privileged, confidential, or exempt from
>> disclosure under applicable law (e.g., personal health
>> information, research data, financial information). Because this e-
>> mail has been sent without encryption, individuals other than the
>> intended recipient may be able to view the information, forward
>> it to others or tamper with the information without the knowledge
>> or consent of the sender. If you are not the intended recipient,
>> or the employee or person responsible for delivering the message
>> to the intended recipient, any dissemination, distribution or
>> copying of the communication is strictly prohibited. If you
>> received the communication in error, please notify the sender
>> immediately by replying to this message and deleting the message
>> and any accompanying files from your system. If, due to the
>> security risks, you do not wish to receive further communications
>> via e-mail, please reply to this message and inform the sender
>> that you do not wish to receive further e-mail from the sender.
>> =====================================================================
>> =========
>> AOL now offers free email to everyone. Find out more about what's
>> free from AOL at AOL.com.
>
|
