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Dear Nathalia,

We had a problem like this on our SP2 some years ago. This may not be that relevant because we have an upright scope with a galvo stage but it turned out that the galvo actually needed recalibrating. We had a Leica engineer recalibrate it and it has been fine since then.

Cheers,

Jacqui

Jacqueline Ross 

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences 
The University of Auckland 
Private Bag 92019 
Auckland, NEW ZEALAND 

Tel: 64 9 373 7599 Ext 87438 
Fax: 64 9 373 7484 

http://www.health.auckland.ac.nz/biru/ 

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Nathalia Holtzman
Sent: 09 November 2007 02:59
To: [log in to unmask]
Subject: Z-drift

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Hi all, 
I need some help. We have a 1 year old Leica SP5 inverted scope and pretty
much all of a sudden we started to get z-drift. Every time you do a time
lapse, no matter what the z-step or time interval, the system does not start
at the top of the sample, it moves down significantly and progressively.
Anything I can do to fix this?

I have called the service rep but would like to fix the problem ASAP. It is
possible that I am the first person to use the system for this application
since we upgraded the software and firmware.

Nathalia

-- 
Nathalia Glickman Holtzman Ph.D.
Department of Biology SB D328
Queens College, CUNY
65-30 Kissena Boulevard
Flushing, NY 11367

Office: 718-997-3678
Lab: 718-997-3517
Fax: 718-997-3445