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Thank you, Tim,
But I don't think this is it. I placed one of the images here:

https://www.dropbox.com/scl/fo/3p8yfmm5rqoo1rqj36bkd/AJOTl2uNKOh6DSXztwO2HWI?rlkey=h771q3h1abhtssv327dng2yre&dl=0

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Tim F
Sent: Thursday, April 11, 2024 5:24 PM
To: [log in to unmask]
Subject: EXT: Re: vesicles

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Hi Michael, just to confirm, if your experience is similar to the video below, do you mean the dark spots? Thanks to Nikon for the vid!

https://youtu.be/Rb-Q3wLsbIk?si=f8GSrHrkTEYmZg1t

I remember thinking those are protein mis-folding aggregates. I don't think you would see endosomes with that kind of contrast, and vesicles are too small at 60nm to see with transmitted light.

Best,


T

Timothy Feinstein, Ph.D.

On Thu, Apr 11, 2024 at 3:53 PM Model, Michael <[log in to unmask]> wrote:

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> Dear List,
>
> If you look at cultured cells, such as HeLa, under bright field, you
> clearly see many vesicles about 1 um. Does anyone know what they are?
> (They are not acidic). The stupidest thing is that I once found a
> paper that investigates them, but now I cannot find that paper!
>
> Mike
>
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