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Dear Confocal listserv,

If anyone is aware of an ImageJ plugin for Number and Molecular 
brightness (N&B) Method, could you please post the link here?

An explanation of N&B can be found in     
http://www.lfd.uci.edu/workshop/2010/files/LFDWorkshop2010-Lecture05.pdf    
as well as various papers by Digman, Gratton et al.  (page 33 of the pdf 
file).

If there is one, could someone please write one in time for me to use 
for a CW-STED demo Aug 22-Sept 6?

If you are feeling ambitious, Cross N&B would be great to have, too. The 
pdf is not explicit, but usually 100 images are acquired. Figure on 
calculating from a stack in ImageJ (two stacks for cross). I'll be happy 
to send you nice data to use in your publication announcing your plugin. 
You are welcome to visit Miami during the demo to acquire however you 
think is best - however, this is the rainy season (and I have no travel 
budget). I anticipate using the demo instrument's HyD detector in photon 
counting mode for most of this.


thanks,

George


-- 


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami