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There is another possibility - use lifetime imaging to distinguish
between dyes with similar spectra but different lifetimes. Carlsson, K.
and Liljeborg, A., 2009. Confocal fluorescence microscopy using
intensity-modulated multiple-wavelength scanning (IMS): evaluation of
results from spectral and lifetime imaging. Proceedings of SPIE 3261, 30
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Olivier Bardot
Sent: Thursday, 10 November 2011 8:52 PM
To: [log in to unmask]
Subject: Re: 5th colour?
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Hello
You can use Chromeo 494. It has a long stokes shift (Em 628nm) that
allow you
to excite two dyes (any 488 et chromeo494) with the same 488 excitation
laser.
Hope this help.
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