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Dear All,

We have a W1 spinning disk with dual cameras. We recently had them aligned,
and upon close inspection, we see that the GFP channel image is about 1 to
2 pixels larger than the RFP image in every corner (we used a 60x
objective, and the cameras are primeBSI express with 6.4um pixels, so the
image is off by 100-200nm in every corner). you can download the two images
as a composite here if you are curious:
https://drive.google.com/file/d/1TWIxwiZF6uf3FspyA96AX6r092PB_102/view?usp=sharing

What would be the best way to solve this issue? Shouldn't the standard
camera mounts enable us to correct this?

Thanks for your help and comments,
Best wishes,

Emmanuel