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Hi Jeremy.

Comet artifact is when HyD becomes excited for much longer time the usual
nanosecond response. Then you can see a comet like signal with tail in
direction of scanning.
On this image is not the best example
https://photos.app.goo.gl/sv9AYVjN22NYBzte7
But you can see a few.
A nice example to have a look on noise without signal, but I did not save
it.
To fix it I was told a few methods.
- To wait. Did not help in my case.
- To take detector out and light it with a flash light. I have not tried it.
- To saturate it with a signal that it just start to beep. I used
convalaria slide and ~15-20 sec of beeps.
In my case the comets disappeared.

As for reason why they come, I do not know.  It could be, as mentioned
here, that someone was severely overloading it, we got a few new  users
last week and I can't babysit them all the time.

Best.
Petro.

On Mon, 15 Apr 2024, 11:10 Jeremy Adler, <[log in to unmask]> wrote:

> *****
> To join or leave the confocal microscopy listserv or to change your email
> address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Petro,
>
> Could you explain what the Hyd comet artefact is/was.
>
> Is the fix, software is so which version.
>
> Best
>
>
> ===============================================
>                     B i o V i s   P l a t f o r m of  Uppsala University
>                    Light & EM microscopy / FlowCytometry & Cell Sorting /
> Image Analysis
> ===============================================
> Jeremy Adler   PhD - Senior research engineer
> Light, Confocal Microscopy, Image Analysis
> E-mail: [log in to unmask]
> 0739 188170
>
> Dag Hammarskjölds v 20
> 751 85 UPPSALA, SWEDEN
> http://biovis.uu.se/
> ===============================================
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[log in to unmask]> On
> Behalf Of Khoroshyy Petro
> Sent: Monday, April 15, 2024 10:53 AM
> To: [log in to unmask]
> Subject: Re: HyD comet artifact?
>
> *****
> To join or leave the confocal microscopy listserv or to change your email
> address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi.
> Sorry.
> It looks like I posted to this list instead of our facility users mailing
> list.
> My apologies.
> Petro.
>
>
> On Mon, 15 Apr 2024, 10:11 Khoroshyy Petro, <[log in to unmask]> wrote:
>
> > Hi everybody.
> > The comet artifact is fixed.
> > If you have it anytime I future.
> > Please let me know.
> > Best regards.
> > Petro.
> >
> > On Sun, 14 Apr 2024, 09:20 Khoroshyy Petro, <[log in to unmask]> wrote:
> >
> >> Hi everybody.
> >> Could you educate me about the comet artifact of HyD and why it happens?
> >> Is there anything I can do to prevent this from happening?
> >> What is the latest information on it?
> >> Thanks.
> >> Petro.
> >>
> >> --
> >> ______________________________
> >> Petro Khoroshyy
> >>
> >
>
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